CAIE A-Level Biology AS 3.2.1 Effects on Enzyme Reaction Rate Questions

A student investigated the hydrolysis of lipid in high-fat milk, using the enzyme lipase.
- $1 \mathrm{~cm}^{3}$ of enzyme solution was added to $10 \mathrm{~cm}^{3}$ of high-fat milk.
- The temperature was kept constant.
- The pH of the reaction mixture was recorded at time 0 minutes and every minute for 20 minutes.

Which statements correctly describe the expected results of this investigation?

1 The product forms more slowly as time proceeds because the concentration of the substrate is decreasing.

2 The pH of the reaction mixture increases rapidly in the first few minutes and then increases less rapidly.

3 The increase in the concentration of product eventually causes the lipase molecules to denature.