CAIE A-Level Biology AS 3.1.4 Enzyme Reaction Investigations Questions

Design and interpret enzyme investigations by identifying variables, controls, measurements and evidence-based conclusions.

Syllabus
2028–2030
Course
Biology 9700
Level
AS

Exam points

  • Design and interpret investigations of enzyme reactions using appropriate variables, controls, measurements and conclusions.

CAIE A-Level Biology AS 3.1.4 Enzyme Reaction Investigations Questions question 1

[Maximum number: 1]

A student investigated the effect of enzyme concentration on the rate of hydrolysis of protein in milk.

When the enzyme and milk were mixed, the protein was hydrolysed and the mixture changed from cloudy to clear.

The student investigated five different enzyme concentrations and recorded the time taken for the mixture to become clear.

What is an appropriate control for this investigation?

A

Carrying out an experiment where the enzyme solution is replaced with water.

B

Carrying out each experiment in a thermostatically controlled water-bath at 35∘C35^{\circ} \mathrm{C}.

C

Repeating each experiment three times for each of the five enzyme concentrations.

D

Using the same volume of enzyme solution for each of the five experiments.

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