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Edexcel IGCSE Biology 5.17B Plant cloning methods

Practise micropropagation stages using explants, sterile technique, agar nutrients and in vitro growth in practical plant-cloning contexts.

Syllabus
First assessment 2019
Course
Biology 4BI1

Exam points

  • describe removing explants with sterile tools and sterilising them with bleach or alcohol
  • complete tissue-culture passages using explant, agar nutrient and clone terminology
  • interpret pH data for shoot growth by linking an optimum to enzyme activity

5.17B Micropropagation process question 1

[Maximum number: 7]

Micropropagation is used to produce plant clones.
The process involves growing explants in vitro.

Question (a)

(a)

State what is meant by the term in vitro.

[ 1 ]

Question (b)

(b)

The explants grow new roots and shoots.

A student investigates the effect of pH on the growth of new shoots.
The table shows the student's results.

Table for Question (b) — Edexcel IGCSE Biology
[ 6 ]

Question (i)

(i)

Describe a procedure the student could use to obtain explants and produce these results.

[ 6 ]
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