Edexcel IGCSE Biology 5.17B Plant cloning methods
Practise micropropagation stages using explants, sterile technique, agar nutrients and in vitro growth in practical plant-cloning contexts.
- Syllabus
- First assessment 2019
- Course
- Biology 4BI1
Practise micropropagation stages using explants, sterile technique, agar nutrients and in vitro growth in practical plant-cloning contexts.
Micropropagation is used to produce plant clones.
The process involves growing explants in vitro.
State what is meant by the term in vitro.
in glass / in test tube / in (Petri) dish
The explants grow new roots and shoots.
A student investigates the effect of pH on the growth of new shoots.
The table shows the student's results.

Describe a procedure the student could use to obtain explants and produce these results.
A description that makes reference to five of the following points:
- use forceps / scalpel / knife / eq to remove explants / pieces from plant ( 1 )
- wash in bleach / hypochlorite / ethanol / alcohol / sterile wipes / eq
- (grow on) agar
- nutrients / minerals / carbohydrates / amino acids / named mineral / eq / growth factors / hormones
- add acid / alkali / buffer ( 1 )
- use several / multiple explants / repeat
- control temperature / carbon dioxide / light ( 1 )
- sterile agar / sterile tubes
- same age plant / same plant / same size of explants / eq
- count number of shoots after same / stated time
Allow sterile cotton wool /
Allow disinfected / ensure sterile conditions / sterilise
equipment