IB Biology SL A2.2.3 Developments in Microscopy Questions

Practise IB Biology SL A2.2.3 by comparing light, electron and immunofluorescence microscopy and explaining how resolution reveals cell structure.

Exam points

Compare light and electron microscopes using resolution, magnification and specimen preparation to explain which observations are possible.
Explain how TEM or SEM resolution and imaging reveal internal ultrastructure or surface detail not visible with a light microscope.
Identify cryogenic electron microscopy or fluorescent-antibody techniques from the sample treatment and imaging purpose.
Identify microscopy and related imaging improvements that provided evidence for cell-membrane structure.

[Maximum number: 1]

The figure shows a transmission electron micrograph of rotavirus particles. Each rotavirus is about 70 nanometres in diameter.

State a reason for using an electron microscope to view this virus rather than a light microscope.

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