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IB Biology SL A2.2.3 Developments in Microscopy Questions

Explain why resolution, as well as magnification, determines visible detail. Compare TEM images of internal ultrastructure with SEM surface detail, and describe how cryogenic electron microscopy, fluorescence and immunofluorescence reveal molecules or specific structures.

Syllabus
First assessment 2025
Course
Biology SL
Level
SL

Exam points

  • Explain why resolution, not magnification alone, determines the detail visible in a microscope image.
  • Distinguish TEM imaging of internal ultrastructure from SEM imaging of surface detail.
  • Describe how cryogenic electron microscopy, fluorescence and immunofluorescence reveal molecules or specific structures.

IB Biology SL A2.2.3 Developments in Microscopy Questions question 1

[Maximum number: 1]

The figure shows a transmission electron micrograph of rotavirus particles. Each rotavirus is about 70 nanometres in diameter.

Figure for Question IB Biology SL A2.2.3 Developments in Microscopy Questions question 1 — IB Biology SL

State a reason for using an electron microscope to view this virus rather than a light microscope.

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