IB Biology SL A2.2.2 Microscopy Skills Questions

Prepare and stain a cell specimen for observation with a light microscope. Measure cells using an eyepiece graticule and stage micrometer, and calculate magnification, actual size and scale bars.

Exam points

Describe how to prepare and stain cell mounts for observation with a light microscope.
Measure cells using an eyepiece graticule and a stage micrometer.
Calculate magnification and actual size, and use or construct scale bars.

[Maximum number: 1]

The image shows a cast of the lower surface of a leaf from a busy Lizzie (Impatiens walleriana) plant as seen under a light microscope with a magnification of $600 \times$ .

Circular microscope field of leaf epidermis showing irregular interlocking cells, dark nuclei and several pairs of guard cells around elongated pores.

State how the magnification of the microscope was calculated.

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IB Biology SL A2.2.2 Microscopy Skills Questions question 1