The genetic code is stored in the order of nitrogenous bases, not in the repeating sugar–phosphate backbone. DNA uses A, T, G and C; RNA uses A, U, G and C. Groups of three bases form codons, and the order of codons carries the instructions for the amino-acid sequence of a polypeptide.
RNA is a single-stranded polynucleotide formed by condensation of nucleotide monomers. Its main types have different roles in protein synthesis:
- mRNA carries a transcript of the DNA instructions to the ribosome.
- tRNA carries amino acids to the mRNA sequence.
- rRNA forms part of the ribosome and provides catalytic activity for joining amino acids.
Answer chain: base sequence → genetic instructions; condensation → RNA polymer; mRNA, tRNA and rRNA → different steps in gene expression.