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AP Biology 1.6 Nucleic Acids Questions

Practise AP Biology 1.6 questions by connecting DNA structure to charge-based fragment movement, backbone chemistry, helix evidence and melting-temperature data.

Syllabus
Effective Fall 2025
Course
AP Biology

Exam points

  • interpret DNA charge and fragment size in a comet-assay or electrophoresis context
  • calculate DNA melting temperature from base composition and identify helix evidence
  • connect the sugar-phosphate backbone, covalent-bond hydrolysis and nucleic acids to DNA structure and function

Covered syllabus points

1.6.A—Describe the structure and function of DNA and RNA

Question 1

[Maximum number: 1]

One method for predicting the average temperature (in °C) at which a short DNA molecule binds with its complement is to calculate a melting temperature ( Tm\mathrm{T}_{\mathrm{m}} ) using the following formula.

Tm=4(G+C)+2( A+T)\mathrm{T}_{\mathrm{m}}=4(\mathrm{G}+\mathrm{C})+2(\mathrm{~A}+\mathrm{T})

Using the formula, what is the predicted Tm\mathrm{T}_{\mathrm{m}} for the short single-stranded DNA molecule shown above?

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