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AP Biology 1.6.A DNA and RNA Structure

Practise AP Biology 1.6.A questions by linking DNA charge, backbone bonds, helix evidence and base composition to molecular structure and laboratory data.

Syllabus
Effective Fall 2025
Course
AP Biology

Exam points

  • interpret DNA charge and fragment size in a comet-assay or electrophoresis context
  • calculate DNA melting temperature from base composition and identify helix evidence

1.6.A—Describe the structure and function of DNA and RNA question 1

[Maximum number: 1]

One method for predicting the average temperature (in °C) at which a short DNA molecule binds with its complement is to calculate a melting temperature ( Tm\mathrm{T}_{\mathrm{m}} ) using the following formula.

Tm=4(G+C)+2( A+T)\mathrm{T}_{\mathrm{m}}=4(\mathrm{G}+\mathrm{C})+2(\mathrm{~A}+\mathrm{T})

Using the formula, what is the predicted Tm\mathrm{T}_{\mathrm{m}} for the short single-stranded DNA molecule shown above?

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