AP Biology 1.3 Macromolecule Reactions Question Bank
Practise AP Biology 1.3 questions by explaining how hydrolysis and dehydration synthesis use water differently to break or build biological polymers.
- Syllabus
- Effective Fall 2025
- Course
- AP Biology
Practise AP Biology 1.3 questions by explaining how hydrolysis and dehydration synthesis use water differently to break or build biological polymers.
To investigate how increases in environmental temperatures affect the metabolism of certain organisms, researchers incubated liver cells from toads at different temperatures and measured two markers of metabolic activity (Table 1): the rate of oxygen consumption and the rate of ATP synthesis.
Describe the role of water in the hydrolysis of ATP.

TABLE 1. RATE OF OXYGEN CONSUMPTION AND ATP SYNTHESIS AT DIFFERENT TEMPERATURES
1 point
Accept one of the following:
- Water is added in the process of cleaving/splitting (a phosphate from) ATP.
- Water breaks down/splits ATP.