Pearson Edexcel IAL Biology 3.16 Mitotic index calculations
Practise calculating mitotic index from counted cells, percentages, microscope images and cell-cycle data tables.
- Syllabus
- First assessment 2019
- Course
- Biology YBI11
- Level
- AS
Practise calculating mitotic index from counted cells, percentages, microscope images and cell-cycle data tables.
Flowering plants produce seeds that can be stored in a seed bank. This is done to conserve the biodiversity of plants.
The longer seeds are stored in seed banks, the less likely they are to germinate. This is due to ageing.
Ageing of seeds can affect the growth of roots when the seeds germinate.
Determining the mitotic index of roots is one way to study growth of roots.
Describe how the mitotic index of roots can be determined.
A description that includes 4 of the following points:
- fixing root tip described (1)
- stain used (1)
- description of maceration / squashing (1)
- view through high power microscope (1)
- count of number of dividing cells and divide by total number of cells (1)
e.g. heating with conc. HCl
Accept magnification quoted >/=100x
(4)
Seeds can be treated to artificially age them.
In an investigation, untreated and treated samples of seeds were germinated and the roots allowed to grow.
Roots of different lengths from the untreated seeds, seeds treated for 4 days and seeds treated for 14 days were selected.
The mitotic index of these roots was determined.
The graph shows the results of this investigation.

There were 14 cells undergoing mitosis in 8 mm roots from seeds treated for 4 days. Calculate the total number of cells that were counted when the mitotic index was determined.
(3)
Total cells counted
An answer that includes the following points.
- read MI from graph (1)
- rearrange equation MI= no. of cells in mitosis ÷ total number of cells to calculate total number of cells (1)
- substitute values in this equation and correct answer (1)
e.g. 5(\%)
total number of cells = no. of cells in mitosis
÷MI
total no. of cells =14÷0.05=280/T=1400÷5=280
correct answer with no working gains 3
accept T = 14 / 5 for 2
(3)