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Pearson Edexcel IAL Biology 3.15 Core Practical 6 root tip squash mitosis

Practise describing root tip squash preparation and using stained microscope samples to observe mitosis stages and mitotic index.

Syllabus
First assessment 2019
Course
Biology YBI11
Level
AS

Exam points

  • describe preparing root tips with acid, stain, squash technique and microscope observation
  • explain why staining, squashing and magnification make chromosomes and mitotic stages visible

3.15—Core Practical 6 - root tip squash mitosis question 1

[Maximum number: 8]

The flow chart shows how garlic root tips can be treated to observe mitosis in the root cells.

Figure for Question 3.15—Core Practical 6 - root tip squash mitosis question 1 — Edexcel A-Level Biology AS

Question (a)

(a)

Give one reason why each of the following steps was taken.
(3)

Step 2

Step 4

Step 5

[ 3 ]

Question (b)

(b)

Describe how the procedure shown in the flow chart could be used to investigate the effect of pH on the mitotic index.

[ 5 ]
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