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Edexcel A-Level Biology AS 2.8 Core Practical 4 Enzyme Catalysed Reaction Rate Questions

Calculate initial rate from early data or a tangent, control substrate and enzyme conditions, and explain concentration, pH and temperature trends through collisions and denaturation.

Syllabus
First assessment 2019
Course
Biology YBI11
Level
AS

Exam points

  • Calculate initial enzyme reaction rate from early data or a tangent with correct units and controlled measurements.
  • Explain substrate or enzyme concentration effects through collisions, active sites, enzyme–substrate complexes and limiting substrate.
  • Interpret pH and temperature investigations through optimum conditions, denaturation and valid practical controls.

Edexcel A-Level Biology AS 2.8 Core Practical 4 Enzyme Catalysed Reaction Rate Questions question 1

[Maximum number: 7]

The enzyme invertase breaks down sucrose into its two component monosaccharides.

Question (a)

(a)

Suggest why the temperature was kept at 25^ C.

[ 2 ]

Question (b)

(b)

Explain why the initial rate of this reaction was determined.

[ 2 ]

Question (c)

(c)

Invertase is used in the food industry. Scientists wanted to increase the efficiency of the enzyme to save time and money.

One way is to immobilise the enzyme by binding it to a surface. This stops the enzyme molecules from moving.

The effect of temperature on immobilised invertase and non-immobilised invertase was investigated. The results are shown in the graph.

Figure for Question (c) — Edexcel A-Level Biology AS

Compare and contrast the effect of temperature on these two forms of invertase.

[ 3 ]
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