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Edexcel A-Level Biology A2 6.1 Culturing Microorganisms and Aseptic Technique Questions

Explain why aseptic technique prevents contamination and infection, describe reliable procedures and control risks when culturing bacteria.

Syllabus
First assessment 2019
Course
Biology YBI11
Level
A2

Exam points

  • explain why aseptic technique prevents contamination and infection in bacterial cultures
  • describe aseptic procedures such as sterilisation, Bunsen updrafts and minimising culture exposure
  • identify culture risks and controls that protect people and preserve valid single-species results

Edexcel A-Level Biology A2 6.1 Culturing Microorganisms and Aseptic Technique Questions question 1

[Maximum number: 4]

Two students, student P and student Q, each cultured bacteria in a liquid medium for 20 hours. The culture conditions used by both students were identical. Every two hours, each student removed a sample of the culture to count the number of bacteria. Each student used a different method to count the number of bacteria in the samples. The graph shows the bacterial growth curve that each student drew using their results. log⁡10\log _{10} number of bacteria per cm3\mathrm{cm}^{3}

Figure for Question Edexcel A-Level Biology A2 6.1 Culturing Microorganisms and Aseptic Technique Questions question 1 — Edexcel A-Level Biology A2

Question (a)

(a)

Both students used aseptic techniques to culture the bacteria.

[ 4 ]

Question (i)

(i)

Explain the importance of using aseptic techniques to culture bacteria.

[ 2 ]

Question (ii)

(ii)

Explain one aseptic technique that should be used when culturing bacteria.

[ 2 ]
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