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Pearson Edexcel IAL Biology 6.1 Culturing microorganisms & aseptic technique

Practise culturing microorganisms by explaining aseptic technique, safe transfer, incubation conditions and contamination control.

Syllabus
First assessment 2019
Course
Biology YBI11
Level
A2

Exam points

  • justify incubation temperature and time using pathogen risk and visible colony growth
  • compare bacteria and fungi culture conditions from tables of agar, temperature and incubation time

6.1—Culturing microorganisms and aseptic technique question 1

[Maximum number: 4]

Two students, student P and student Q, each cultured bacteria in a liquid medium for 20 hours. The culture conditions used by both students were identical. Every two hours, each student removed a sample of the culture to count the number of bacteria. Each student used a different method to count the number of bacteria in the samples. The graph shows the bacterial growth curve that each student drew using their results. log10\log _{10} number of bacteria per cm3\mathrm{cm}^{3}

Figure for Question 6.1—Culturing microorganisms and aseptic technique question 1 — Edexcel A-Level Biology A2

Question (a)

(a)

Both students used aseptic techniques to culture the bacteria.

[ 4 ]

Question (i)

(i)

Explain the importance of using aseptic techniques to culture bacteria.

[ 2 ]

Question (ii)

(ii)

Explain one aseptic technique that should be used when culturing bacteria.

[ 2 ]
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