CAIE A-Level Biology AS 11.2.2 Hybridoma Method Questions
Explain the hybridoma method for producing monoclonal antibodies using evidence from each question and mark scheme.
- Syllabus
- 2028–2030
- Course
- Biology 9700
- Level
- AS
Exam points
- Explain the hybridoma method for producing monoclonal antibodies.
CAIE A-Level Biology AS 11.2.2 Hybridoma Method Questions question 1
[Maximum number: 3]
Rheumatoid arthritis (RA) is a disease of the joints in the human body.
Outline how monoclonal antibody against TNF- α is produced.
1 TNF- α / antigen, introduced into, mice/ small mammals/ named ;
2 (antibody-producing), B-cells / B-lymphocytes / plasma cells / splenocytes, isolated (from spleen) ;
A produced 3 fused with myeloma cells ;
4 using fusogen/PEG ;
5 hybridoma cells formed ;
6 ref. to screening / testing, for hybridoma producing desired antibody ;
7 ref. to scaling up/large-scale production ;
8 AVP ;
e.g. HAT medium for, hybridoma growth/inhibiting myeloma growth
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