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CAIE A-Level Biology 1.1.4 Microscope Measurement Tools Question Bank

Practise microscope-measurement questions by calibrating eyepiece graticules with stage micrometers, applying the same magnification and converting units.

Syllabus
2028–2030
Course
Biology 9700
Level
AS

Exam points

  • calibrate eyepiece units by matching graticule divisions to a stage micrometer scale
  • apply the same objective-lens calibration to specimen width, diameter or field measurements
  • convert mm, µm and nm when calculating cell size, growth rate or field-of-view area

1.1.4—Microscope measurement tools question 1

[Maximum number: 1]

Smooth muscle is a tissue composed of smooth muscle cells. The cells contain cytoplasm packed with proteins that are involved in contraction and relaxation.

When viewed in longitudinal section (LS), smooth muscle cells are elongated and taper at both ends. This is known as a fusiform shape. Each cell has a central nucleus, which also appears elongated.

Fig. 1.1 is a diagram of a smooth muscle cell to show the fusiform shape.

Fig. 1.1

Fig. 1.1

A student used a microscope fitted with a calibrated eyepiece graticule to estimate that the length of one smooth muscle cell was 250 micrometres (μm)(\mu \mathrm{m}).

Name the type of microscope slide that the student used to calibrate the eyepiece graticule.

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