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CAIE A-Level Biology AS 1.1 The Microscope in Cell Studies Questions

Use real CAIE A-Level Biology questions to calculate magnification and actual size, calibrate graticules, interpret microscopy resolution, and compare light, SEM and TEM evidence.

Syllabus
2028–2030
Course
Biology 9700
Level
AS

Exam points

  • Calculate magnification, image size or actual size using M = image size ÷ actual size, scale bars and consistent units.
  • Calibrate an eyepiece graticule against a stage micrometer, then measure structures and convert between mm, µm and nm.
  • Distinguish magnification from resolution, relate wavelength to visible detail, and choose light, SEM or TEM for the required structure or specimen.

Covered syllabus points

1.1.1—Temporary cell preparations1.1.2—Cell drawings1.1.3—Magnification and actual size1.1.4—Microscope measurement tools1.1.5—Resolution and magnification

Question 1

[Maximum number: 1]

A prokaryotic cell which is 1μ m1 \mu \mathrm{~m} in diameter is magnified 50000 times in an electron micrograph.

What is the diameter of the cell in the electron micrograph?

A

5×10−1 mm5 \times 10^{-1} \mathrm{~mm}

B

5×100 mm5 \times 10^{0} \mathrm{~mm}

C

5×101 mm5 \times 10^{1} \mathrm{~mm}

D

5×102 mm5 \times 10^{2} \mathrm{~mm}

Question 2

[Maximum number: 1]

An eyepiece graticule can be calibrated using a stage micrometer.
What is the correct reason why an eyepiece graticule is calibrated?

A

An eyepiece graticule can be used to make measurements.

B

An eyepiece graticule is magnified by the objective lens.

C

An eyepiece graticule magnifies the specimen.

D

An eyepiece graticule makes comparisons.

Question 3

[Maximum number: 1]

Which statement explains why it is necessary to use an electron microscope to see the cristae of a mitochondrion?

A

The magnification of the electron microscope is greater than that of the light microscope.

B

The membranes of the cristae are separated by a distance greater than 200 nm .

C

The maximum resolution of a microscope using visible light is too low.

D

The wavelength of an electron beam is longer than the wavelength of visible light.

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