CAIE A-Level Biology A2 15.2.3 Gibberellin Role in Barley Germination Questions
Explain the role of gibberellin in barley germination using evidence from each question and mark scheme.
- Syllabus
- 2028–2030
- Course
- Biology 9700
- Level
- A2
Explain the role of gibberellin in barley germination using evidence from each question and mark scheme.
An investigation was carried out to study the effect of different intensities of blue light on the percentage germination of barley seeds. Barley seeds were exposed to blue light for a period of seven days. All other variables were kept constant.
The results are shown in Table 9.1.
Table 9.1
The effect of blue light on the concentration of abscisic acid (ABA) was also investigated. ABA concentration was measured at intervals over seven days in barley seeds exposed to blue light at an intensity of 57 arbitrary units.
The results are shown in Table 9.2.
Table 9.2
For comparison, in the dark the concentration of ABA in barley seeds fell from 100 au at the start (day 0 ) to 45 au on day 1 and did not increase from day 1 to day 7.
ABA is thought to affect gibberellin synthesis or activity.
Using the information in Table 9.1 and Table 9.2, describe the effect of blue light on the germination of barley seeds and suggest an explanation for this effect.
(b) After germination, auxin is important in the growth of barley plants.
Describe and explain the role of auxin in cell elongation.
9(a) Any four from:
1. blue light decreases germination / AW ;
2. as intensity of blue light increases, percentage germination decreases ;
3. data quote:
4. blue light increases the concentration of ABA ;
5. ABA inhibits gibberellin synthesis / activity ;
6. any further valid suggestion of ABA action, e.g. DELLA not broken down / promotes dormancy / stops gene expression ;
9(b) Any seven from:
1. auxin binds to receptor on the cell surface membrane ;
2. stimulates proton pumps ;
3. protons move from cytoplasm / cell to cell wall ;
4. pH of cell wall decreases ;
5. expansins activated ;
6. loosens linkage between cellulose microfibrils ;
7. by breaking hydrogen bonds ;
Ignore weakening bonds.
8. K+ channels open ;
9. K+ diffuse into cytoplasm / cell ;
10. water potential of cytoplasm / cell decreases ;
11. water enters by osmosis ;
12. turgor pressure increases / volume of cell increases ;
| light intensity / arbitrary units (au) | percentage germination |
|---|---|
| 0 (dark) | 98.0 |
| 36 | 76.9 |
| 48 | 45.0 |
| 57 | 14.7 |