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CAIE A-Level Biology A2 13.2.3 Chloroplast Suspension Investigation Questions

Explain chloroplast suspension investigations using evidence from each question and mark scheme.

Syllabus
2028–2030
Course
Biology 9700
Level
A2

Exam points

  • Explain the chloroplast suspension investigation.

CAIE A-Level Biology A2 13.2.3 Chloroplast Suspension Investigation Questions question 1

[Maximum number: 3]

Question (a)

(a)

Chloroplasts isolated from leaf palisade cells can still function if they are suspended in a buffer solution. The buffer solution has the same water potential as the chloroplasts.

The dye DCPIP is a hydrogen acceptor that changes colour from blue to colourless when it becomes reduced.

Three test tubes were set up as shown in Table 7.2 and left for 20 minutes to allow any colour change to occur. The results are also shown in Table 7.2.

Table 7.2

Table 7.2

[ 3 ]

Question (i)

(i)

Explain the results for test-tube 2.

[ 2 ]

Question (ii)

(ii)

Test-tube 1 is a control tube. Explain why test-tube 1 was included in the investigation.

[ 1 ]
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