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CAIE A-Level Biology 13.2.3 Chloroplast Suspension Investigations

Practise testing light intensity or wavelength with chloroplasts and DCPIP by controlling pH and temperature, using dark controls and measuring reduction by colour loss.

Syllabus
2028–2030
Course
Biology 9700
Level
A2

Exam points

  • prepare buffered chloroplast suspension and mix equal amounts with oxidised blue DCPIP
  • vary light intensity or wavelength while controlling temperature and exposure time
  • measure absorbance or decolourisation and use dark or chloroplast-free controls

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