CAIE A-Level Biology A2 13.2.3 Chloroplast Suspension Investigation Questions

The light-dependent stage of photosynthesis occurs within chloroplasts. In this stage, electrons are emitted from the chlorophyll a molecules and passed to electron acceptors.

If a redox indicator, such as DCPIP, is added to a suspension of illuminated chloroplasts, electrons will be transferred to DCPIP, causing the colour of the DCPIP to change from blue to colourless.

A student investigated the effect of the wavelength of light (colour of light) on the rate of photosynthesis.
- DCPIP was added to three colorimeter tubes, each containing a suspension of chloroplasts. The chloroplast suspensions were kept in the dark until required.
- The colorimeter tubes were each exposed to light of a different colour: red, blue or green. The intensity of light was the same for all tubes, and each was exposed to light for four minutes. All other conditions were kept the same.
- The absorbance of each chloroplast suspension was measured at one-minute intervals using a colorimeter.

The results are shown in Fig. 7.1.

(i) Explain why the chloroplast suspensions were kept in the dark until required.
(ii) Describe the results shown in Fig. 7.1.
(iii) With reference to the light-dependent stage of photosynthesis, explain the differences between the results shown in Fig. 7.1 for red light and for green light.
(b) Changes in the atmospheric carbon dioxide concentration, light intensity and temperature can affect the rate of photosynthesis. These three factors directly affect different processes of photosynthesis.

Complete Table 7.1 using a tick $(\checkmark)$ to identify the processes that can be directly affected by each factor or a cross ( X ) to identify the processes that are not directly affected by each factor.

Indirect effects where a change in the rate of one process affects the rate of a different process should not be considered.

A tick or a cross must be placed in the final column of every row.