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AP Biology 6.8 Biotechnology Questions

Practise AP Biology 6.8 questions by using genetic engineering techniques to analyze DNA, amplify sequences, transform bacteria and compare samples.

Syllabus
Effective Fall 2025
Course
AP Biology

Exam points

  • interpret gel electrophoresis and restriction-fragment patterns to compare alleles and nucleic-acid samples
  • explain PCR using denaturation, primer annealing, extension and heat-stable DNA polymerase
  • use plasmid transformation, selectable markers, sequencing and complementary probes to analyze or manipulate genes
  • interpret qPCR expression measures and evaluate eDNA specificity, fragment length and false-positive evidence

Covered syllabus points

6.8.A—Explain the use of genetic engineering techniques in analyzing or manipulating DNA

Question 1

[Maximum number: 1]

PCR is a technique used to copy a target DNA sequence. Researchers often use DNA polymerase from Thermus aquaticus, a bacteria species that lives around hydrothermal vents.

Figure for Question 1 — AP Biology

Which of the following best explains why the polymerase from the species T. aquaticus is often used for PCR ?

A

T. aquaticus polymerase has an optimal temperature of 68°C.

B

T. aquaticus polymerase does not denature at high temperatures.

C

T. aquaticus polymerase can be used more than once without degrading.

D

T. aquaticus polymerase adds nucleotides to both the 3′3^{\prime} and 5′5^{\prime} ends of DNA.

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