AP Biology 6.8: Biotechnology

Practise AP Biology 6.8 questions by using genetic engineering techniques to analyze DNA, amplify sequences, transform bacteria and compare samples.

Exam points

interpret gel electrophoresis and restriction-fragment patterns to compare alleles and nucleic-acid samples
explain PCR using denaturation, primer annealing, extension and heat-stable DNA polymerase
use plasmid transformation, selectable markers, sequencing and complementary probes to analyze or manipulate genes
interpret qPCR expression measures and evaluate eDNA specificity, fragment length and false-positive evidence

Covered syllabus points

6.8.A—Explain the use of genetic engineering techniques in analyzing or manipulating DNA

[Maximum number: 1]

PCR is a technique used to copy a target DNA sequence. Researchers often use DNA polymerase from Thermus aquaticus, a bacteria species that lives around hydrothermal vents.

Figure for Question 6.8 Biotechnology question 1 — AP Biology

Which of the following best explains why the polymerase from the species T. aquaticus is often used for PCR ?

T. aquaticus polymerase has an optimal temperature of 68°C.

T. aquaticus polymerase does not denature at high temperatures.

T. aquaticus polymerase can be used more than once without degrading.

T. aquaticus polymerase adds nucleotides to both the $3^{\prime}$ and $5^{\prime}$ ends of DNA.

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Covered syllabus points

6.8 Biotechnology question 1