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AP Biology 6.8 A Explain the Use of Genetic Engineering Techniques in Analyzing or Manipulating Dna Questions

Practise AP Biology 6.8.A questions by interpreting gel electrophoresis, PCR, bacterial transformation, DNA sequencing and DNA fingerprinting evidence.

Syllabus
Effective Fall 2025
Course
AP Biology

Exam points

  • interpret gel electrophoresis bands by connecting fragment size and charge with DNA sample comparisons
  • explain PCR through denaturation, primer annealing, extension and use of heat-stable DNA polymerase
  • use bacterial transformation, DNA sequencing or DNA fingerprints to analyze or manipulate genetic material

AP Biology 6.8 A Explain the Use of Genetic Engineering Techniques in Analyzing or Manipulating Dna Questions question 1

[Maximum number: 1]

PCR is a technique used to copy a target DNA sequence. Researchers often use DNA polymerase from Thermus aquaticus, a bacteria species that lives around hydrothermal vents.

Figure for Question AP Biology 6.8 A Explain the Use of Genetic Engineering Techniques in Analyzing or Manipulating Dna Questions question 1 — AP Biology

Which of the following best explains why the polymerase from the species T. aquaticus is often used for PCR ?

A

T. aquaticus polymerase has an optimal temperature of 68°C.

B

T. aquaticus polymerase does not denature at high temperatures.

C

T. aquaticus polymerase can be used more than once without degrading.

D

T. aquaticus polymerase adds nucleotides to both the 3′3^{\prime} and 5′5^{\prime} ends of DNA.

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