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AP Biology 6.8.A: Genetic Engineering Techniques

Practise AP Biology 6.8.A questions by interpreting gel electrophoresis, PCR, bacterial transformation, DNA sequencing and DNA fingerprinting evidence.

Syllabus
Effective Fall 2025
Course
AP Biology

Exam points

  • interpret gel electrophoresis bands by connecting fragment size and charge with DNA sample comparisons
  • explain PCR through denaturation, primer annealing, extension and use of heat-stable DNA polymerase
  • use bacterial transformation, DNA sequencing or DNA fingerprints to analyze or manipulate genetic material

6.8.A—Explain the use of genetic engineering techniques in analyzing or manipulating DNA question 1

[Maximum number: 1]

PCR is a technique used to copy a target DNA sequence. Researchers often use DNA polymerase from Thermus aquaticus, a bacteria species that lives around hydrothermal vents.

Figure for Question 6.8.A—Explain the use of genetic engineering techniques in analyzing or manipulating DNA question 1 — AP Biology

Which of the following best explains why the polymerase from the species T. aquaticus is often used for PCR ?

A

T. aquaticus polymerase has an optimal temperature of 68°C.

B

T. aquaticus polymerase does not denature at high temperatures.

C

T. aquaticus polymerase can be used more than once without degrading.

D

T. aquaticus polymerase adds nucleotides to both the 3′3^{\prime} and 5′5^{\prime} ends of DNA.

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