Question 1
Louis investigated the effect of sleep disturbance on appetite. He conducted his investigation using nine rats. Louis set an alarm bell to ring at fixed one hour intervals over a ten-day period to disturb the rats.
Louis provided 25 grams of food to each rat at 09:00hrs each day. He recorded the amount of food remaining at 19:00hrs when he removed the food from the cage.
A control group of four rats that were not deprived of sleep were used as a comparison for his results.
Describe three ethical considerations Louis should have considered when he used animals during his investigation.
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