Trace gene isolation, restriction-enzyme cutting, ligation, host transformation and expression, then compare GM bacteria, animal or insect cells for yield, safety and processing.
Small variations in DNA sequences and activation of genes can explain why individuals respond differently to diseases and the drugs used to treat them.
Prader-Willi syndrome is an inherited genetic disorder that involves changes on chromosome 15. The chromosome inherited from the father has genetic deletions. The chromosome inherited from the mother has DNA methylated bases. The diagram shows these chromosomes for an individual with Prader-Willi syndrome. region of DNA methylation
region of genetic deletion
The symptoms of Prader-Willi syndrome can be treated using human growth hormone (HGH). This hormone is a protein produced by the pituitary gland. Describe how Escherichia coli bacteria can be used to produce human growth hormone.