any three from:
S1 sympatric (speciation) ;
S2 different mutations ;
S3 gave, (named) phenotypic / behavioural, differences ;
S4 which gave behavioural, isolation / separation ;
S5 (leading to) inability to reproduce together / reproductive isolation / genetic isolation / no gene flow between them ;
or
any three from:
A1 allopatric (speciation) ;
A2 geographical, separation / barrier, in the past ;
A3 different, mutations / selection pressures ;
A4 gave, (named) phenotypic / behavioural, differences ;
A5 inability to reproduce together / reproductive isolation / genetic isolation / no gene flow between them, after populations re-join ;