CAIE A-Level Biology A2 13.1.6 Pigment Chromatography Questions
Explain pigment chromatography in photosynthesis using evidence from each question and mark scheme.
- Syllabus
- 2028–2030
- Course
- Biology 9700
- Level
- A2
Explain pigment chromatography in photosynthesis using evidence from each question and mark scheme.
Fig. 1.1 shows a transmission electron micrograph of part of a chloroplast.
Fig. 1.1
Membranes of the type labelled C in Fig. 1.1 were made into a liquid extract. Chromatography was then used to separate and identify the coloured components (pigments) in this extract. The resulting chromatogram showed that these membranes contain a yellow pigment, an orange pigment, a green-brown pigment and two different green pigments.
Describe how you would carry out chromatography to separate and identify the coloured pigments in the liquid extract of C.
any four from:
1 spot / extract, placed on, pencil line / base line / line of origin ;
2 repeat / concentrate, spot / extract ;
3 end / base, of paper, suspended / placed, in (named) solvent ;
4 mark the solvent front after, some time / paper removed / solvent moves ;
5 Rf value = distance moved by, spot / pigment / component / solute ÷ distance moved by solvent (front) ;
6 compare (Rf) with known Rf values to identify, constituents / pigments ;
7 detail of method ;