Edexcel IGCSE Biology Investigation Variables

Pineapple juice contains a protease called bromelain.
A student uses this method to investigate the digestion of solid gelatine protein by bromelain.
- place solid gelatine protein into a test tube up to a height of 5 cm
- mix $5 \mathrm{~cm}^{3}$ pineapple juice with $1 \mathrm{~cm}^{3}$ of pH 4 buffer
- place $1 \mathrm{~cm}^{3}$ of the pineapple juice and buffer solution on top of the gelatine
- leave for one hour in a water bath set to $37^{\circ} \mathrm{C}$
- measure the height of the solid gelatine and use it to calculate the volume of gelatine that has been digested

Repeat the method three more times.
The diagram shows part of the student's method.

The student repeats the investigation with different pH buffers.

The table shows their results.

Give two variables the student should control.

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