A student uses this method to investigate the nitrogen cycle.
- take two samples of soil, each of mass 100 g
- sterilise one sample of soil by heating at 100∘C for one hour
- place the sterilised and unsterilised samples into separate filter funnels
- pour 25 cm3 of water through each soil sample and collect the filtrate in a test tube
- test each filtrate for nitrates
- pour water through each soil sample for 5 minutes
- pour another 25 cm3 of water through each soil sample and collect the filtrate in a test tube
- test each filtrate for nitrates
- add 1 cm3 of a solution of ammonium salts to each soil and leave for three days
- pour 25 cm3 of water through each soil sample again and collect the filtrate in a test tube
- test each filtrate for nitrates
The diagram shows the student's apparatus.