Edexcel IGCSE Biology Accuracy and Validity
Practise Edexcel IGCSE Biology accuracy and validity by explaining subjective observations, timing delays and limits in biological investigations.
- Syllabus
- Issue 3 (2024)
- Course
- Biology 4BI1
Practise Edexcel IGCSE Biology accuracy and validity by explaining subjective observations, timing delays and limits in biological investigations.
Apples contain an enzyme called phenol oxidase.
When apple tissue is exposed to oxygen in the air, this enzyme turns the apple tissue brown.
A student uses this method to investigate the effect of pH on the time taken for apple tissue to turn brown.
- mash an apple to produce a pulp
- place 25 g of this pulp into a beaker
- mix a pH 7 buffer with the pulp to maintain the pH
- check the colour of the pulp every five minutes
- record the time when the pulp turns brown
The student repeats this method using pH buffers of 3, 5, 9, and 11.
The graph shows the student's results.

Explain how the student could modify this method to give a more accurate measure of the time taken for the apple pulp to turn brown.
An explanation that makes reference to two of the following points:
Accept use a reference colour / eq
Accept depends on person viewing / eq
2. (because) colour change is subjective / to standardise the end colour / so colour
is always same / eq