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CAIE IGCSE Chemistry: Colourless-Substance Chromatography Question Bank

Practise separating colourless soluble mixtures by chromatography and explaining how locating agents make amino acid spots visible.

Syllabus
2026–2028
Course
Chemistry 0620

Exam points

  • state that colourless amino acids can be separated by chromatography and detected with a locating agent
  • link a locating agent to coloured spots on a dried chromatogram, not to dissolving or preserving amino acids
  • use Rf after separation and detection to help identify colourless substances

12.3.3—Paper chromatography is used to question 1

[Maximum number: 3]

Proteins are natural polymers.

Proteins are broken down into amino acids. The process is similar to how complex carbohydrates are broken down to give simple sugars.

Amino acids are colourless.

A sample containing a mixture of amino acids is separated. Each amino acid is detected and identified.
- Name the process used to separate the amino acids.
- Name the type of substance used to detect the amino acids.
- Give the symbol of the value used to determine the identity of each amino acid after separation and detection.

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