4.4.4—Biochemical oxygen demand (BOD)

Syllabus
First assessment 2026
Objective
4.4.4
Level
HL

Read BOD as Oxygen Used

BOD is dissolved oxygen consumed by microbes decomposing biodegradable organic matter under a specified test condition.

In the five-day, 20°C test, BOD = initial DO − final DO. More biodegradable material means more microbial respiration and less oxygen left for aquatic life.

If DO falls from 8 to 2 mg/L, BOD is 6 mg O₂/L.

High BOD does not mean the water contains abundant oxygen: it means microbes used oxygen rapidly, leaving aquatic organisms under greater oxygen stress.

BOD is oxygen consumed, not oxygen concentration left in the river.