a. amino acid sequence of the protein from each organism is compared;
b. changes in (base/amino acid) sequence accumulate over time;
c. due to mutation;
d. positive correlation between number of (base/amino acid) differences between (two) species and time they diverged from a common ancestor
OR
fewer the (base/amino acid) differences, the less time since diverged from a common ancestor / more closely related;
e. cladograms illustrate probable sequence of divergence/evolutionary origins;
f. accumulation of mutations can be used as a molecular clock/allows time since evolutionary divergence to be estimated;
4 max