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IB Biology HL 2.2.3 Developments in Microscopy

Practise IB Biology HL 2.2.3 by comparing microscopy methods and interpreting immunofluorescence evidence for specific cell structures.

Syllabus
First assessment 2025
Course
Biology HL
Level
HL

Exam points

  • Compare light and electron microscopes using resolution, magnification, living samples and image quality.
  • Explain how immunofluorescence uses fluorescent antibodies to identify specific cell components.

A2.2.3—Developments in microscopy question 1

[Maximum number: 1]

The images of the radiolarian, a single-celled marine organism, were produced using a light microscope (left) and a scanning electron microscope (right).

Figure for Question A2.2.3—Developments in microscopy question 1 — IB Biology HL

What is a reason for the difference in quality of these images?

A

Light cannot pass through the specimen.

B

Higher magnification can be achieved with the electron microscope.

C

The resolution of the electron microscope is higher.

D

Samples are stained with methylene blue when viewed with the light microscope.

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