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IB Biology HL A2.2.3 Developments in Microscopy Questions

Explain why resolution, as well as magnification, determines visible detail. Compare TEM images of internal ultrastructure with SEM surface detail, and describe how cryogenic electron microscopy, fluorescence and immunofluorescence reveal molecules or specific structures.

Syllabus
First assessment 2025
Course
Biology HL
Level
HL

Exam points

  • Explain why resolution, not magnification alone, determines the detail visible in a microscope image.
  • Distinguish TEM imaging of internal ultrastructure from SEM imaging of surface detail.
  • Describe how cryogenic electron microscopy, fluorescence and immunofluorescence reveal molecules or specific structures.

IB Biology HL A2.2.3 Developments in Microscopy Questions question 1

[Maximum number: 1]

The images of the radiolarian, a single-celled marine organism, were produced using a light microscope (left) and a scanning electron microscope (right).

Figure for Question IB Biology HL A2.2.3 Developments in Microscopy Questions question 1 — IB Biology HL

What is a reason for the difference in quality of these images?

A

Light cannot pass through the specimen.

B

Higher magnification can be achieved with the electron microscope.

C

The resolution of the electron microscope is higher.

D

Samples are stained with methylene blue when viewed with the light microscope.

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