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IB Biology HL A2.2.3 Developments in Microscopy Questions

Practise IB Biology HL A2.2.3 by comparing light, electron and immunofluorescence microscopy and explaining how resolution reveals cell structure.

Syllabus
First assessment 2025
Course
Biology HL
Level
HL

Exam points

  • Compare light and electron microscopes using resolution, magnification and specimen preparation to explain which observations are possible.
  • Explain how TEM or SEM resolution and imaging reveal internal ultrastructure or surface detail not visible with a light microscope.
  • Identify cryogenic electron microscopy or fluorescent-antibody techniques from the sample treatment and imaging purpose.
  • Identify microscopy and related imaging improvements that provided evidence for cell-membrane structure.

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Study A2.2.3—Developments in microscopy conceptsOpen the corresponding Concept lesson for explanations, examples and exam connections.

IB Biology HL A2.2.3 Developments in Microscopy Questions question 1

[Maximum number: 1]

The images of the radiolarian, a single-celled marine organism, were produced using a light microscope (left) and a scanning electron microscope (right).

Figure for Question IB Biology HL A2.2.3 Developments in Microscopy Questions question 1 — IB Biology HL

What is a reason for the difference in quality of these images?

A

Light cannot pass through the specimen.

B

Higher magnification can be achieved with the electron microscope.

C

The resolution of the electron microscope is higher.

D

Samples are stained with methylene blue when viewed with the light microscope.

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