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Edexcel A-Level Biology A2 5.7 Chloroplast Pigment Chromatography Questions

Compare pigment solubility and solvent extraction, identify pigments from chromatograms and calculate Rf values from measured distances.

Syllabus
First assessment 2019
Course
Biology YBI11
Level
A2

Exam points

  • separate chloroplast pigments by chromatography and compare their solubility
  • identify pigments from chromatogram distances and calculate Rf values
  • evaluate solvent extraction methods and explain different pigment concentrations

Edexcel A-Level Biology A2 5.7 Chloroplast Pigment Chromatography Questions question 1

[Maximum number: 2]

Chloroplasts are involved in both the light-dependent reactions and light-independent reactions of photosynthesis.

Question (a)

(a)

Chloroplast pigments can be separated and then identified by their Rf values.

[ 2 ]

Question (i)

(i)

Which process can be used to separate chloroplast pigments?

A

chromatography

B

dendrochronology

C

osmosis

D

PCR

[ 1 ]

Question (ii)

(ii)

The diagram shows separated chloroplast pigments, J and K.

Figure for Question (ii) — Edexcel A-Level Biology A2

What is the Rf value for chloroplast pigment J ?
□ A0.625
□ B0.800
□ C 0.830
□ D 1.714

[ 1 ]
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