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Edexcel A-Level Biology A2 5.7 Chloroplast Pigment Chromatography Questions

Mark the origin and solvent front, measure both distances from the same baseline, calculate Rf and use spot position or colour to support pigment comparisons.

Syllabus
First assessment 2019
Course
Biology YBI11
Level
A2

Exam points

  • Describe chromatography with an origin, solvent movement and separated pigment spots.
  • Calculate Rf accurately from pigment distance divided by solvent-front distance.
  • Compare pigment profiles using matched and unmatched spots or reference Rf values.

Edexcel A-Level Biology A2 5.7 Chloroplast Pigment Chromatography Questions question 1

[Maximum number: 2]

Chloroplasts are involved in both the light-dependent reactions and light-independent reactions of photosynthesis.

Question (a)

(a)

Chloroplast pigments can be separated and then identified by their Rf values.

[ 2 ]

Question (i)

(i)

Which process can be used to separate chloroplast pigments?

A

chromatography

B

dendrochronology

C

osmosis

D

PCR

[ 1 ]

Question (ii)

(ii)

The diagram shows separated chloroplast pigments, J and K.

Figure for Question (ii) — Edexcel A-Level Biology A2

What is the Rf value for chloroplast pigment J ?
□ A0.625
□ B0.800
□ C 0.830
□ D 1.714

[ 1 ]
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