CAIE A-Level Biology A2 16.3.3 Genetic Control in Prokaryotes Questions
Explain genetic control in prokaryotes using evidence from each question and mark scheme.
- Syllabus
- 2028–2030
- Course
- Biology 9700
- Level
- A2
Explain genetic control in prokaryotes using evidence from each question and mark scheme.
Gene expression in a cell is controlled. When a gene is expressed (switched on), the gene is transcribed. When a gene is not expressed (switched off), the gene is not transcribed.
Environmental changes can cause some genes to be switched on or switched off.
An example of control of gene expression in prokaryotes is regulation in the lac operon.
The lac operon is a length of DNA that is made up of different parts.
Fig. 3.1 shows a simple diagram representing the lacI (regulatory) gene and the lac operon.
Fig. 3.1
Outline the main features of the lac operon.
The operon has:
- promoter ;
- operator ;
- three structural genes / named three structural genes: lacZ, lacY and lacA ;
Ignore regulatory genes / order of named parts.
Explain the role of the l a c I gene in the regulation of the lac operon.
any four from:
lacI gene
1 is always expressed ;
2 controls (structural) gene expression ;
3 codes for the repressor (protein) ;
4 repressor, binds to the operator / blocks the promoter ;
5 prevents, (structural) gene expression / RNA polymerase binding to promoter ;
6 lactose / allolactose, binds to repressor ;
7 (so) repressor cannot bind to operator / promoter unblocked / gene expression can occur ;