18. Classification, Biodiversity and Conservation

Syllabus
9700–2028–2029
Section
18
Level
A2

18.1 Classification

Syllabus
9700–2028–2029
Topic
18.1
Level
A2

Species concepts use different evidence

A species concept is a criterion for deciding whether organisms belong to the same species. Biological, morphological and ecological concepts use different evidence, so they can lead to different conclusions about the same organisms.

Concept Same species when… Important limit
Biological they can interbreed and produce fertile offspring difficult or impossible to apply to fossils, asexual organisms or populations that cannot be tested together
Morphological they share the observable structural features used to define the group variation within a species and very similar-looking separate species can mislead
Ecological they occupy the same ecological niche: they use resources and interact with the environment in the same way sharing a habitat alone is not enough; different niches can exist in the same place

For example, two bear populations may look different and use different habitats and foods, supporting separate morphological and ecological classifications. If they interbreed and produce fertile offspring, the biological concept instead supports treating them as the same species. The answer depends on the stated concept and evidence.

The ecological species concept is based on niche, not simply on organisms living in the same area at the same time. No one concept works perfectly in every case.

The three domains reflect deep evolutionary divisions

The three domains are Bacteria, Archaea and Eukarya. Domain is the highest taxonomic rank, and grouping organisms into domains helps represent deep evolutionary divisions.

Bacteria and Archaea contain prokaryotic cells: they lack a nucleus. Eukarya contains eukaryotic cells: genetic material is enclosed in a nucleus and the cell has compartmentalised structures, including membrane-bound organelles.

Cell type is an important first boundary, but it is not enough to classify all prokaryotes. Molecular evidence, especially comparisons of RNA genes, supports separating the two prokaryotic domains and recognising their deeper relationships.

Use the classification in this order:

  1. Ask whether the cell is prokaryotic or eukaryotic.
  2. If prokaryotic, keep Bacteria and Archaea as separate possible domains rather than treating “prokaryote” as a domain.
  3. Use molecular evidence alongside cell organisation before assigning the domain.
  4. State the result as Bacteria, Archaea or Eukarya; detailed Archaea–Bacteria differences and lower Eukaryotic kingdoms are separate questions.

A simple cell shape cannot by itself identify the domain. “Prokaryote” describes cell organisation, whereas Bacteria and Archaea are separate domains; Eukarya is the eukaryotic domain. Viruses are outside this cellular three-domain classification.

Three features separate Archaea from Bacteria

Archaea and Bacteria are both prokaryotes: their cells lack a nucleus and membrane-bound organelles. They are separate domains because they differ in membrane lipids, ribosomal RNA and cell-wall composition.

Feature Bacteria Archaea
Membrane lipids fatty acids are ester-linked to glycerol branched hydrocarbon chains are ether-linked to glycerol
Ribosomal RNA one characteristic prokaryotic type distinct rRNA types; sequences and ribosomal subunits show similarities to Eukarya
Cell wall contains peptidoglycan does not contain peptidoglycan; composition is different

First establish that the organism is prokaryotic, then compare evidence from all three features. Lack of a nucleus narrows the classification to Archaea or Bacteria but does not decide between them.

Living in an extreme environment is not the definition of Archaea, and not all Archaea are extremophiles. Domain classification depends on cellular and molecular evidence.

Eukaryotic taxonomy is nested

Taxonomy places similar organisms into nested ranks. Within Eukarya, the broad domain contains kingdoms, and each lower rank contains a smaller, more similar group.

Read the hierarchy from broad to specific:
Domain: Eukarya
→ Kingdom
→ Phylum
→ Class
→ Order
→ Family
→ Genus
→ Species

At a higher rank, the group contains more organisms with less similarity between them. Moving down the nested hierarchy applies more shared classification evidence, so the group contains fewer organisms with greater similarity. Species is the lowest rank in this sequence.

Use the hierarchy in this order:

  1. Confirm the organism belongs to Eukarya.
  2. Place it in a kingdom, then progressively narrower phylum, class, order, family, genus and species groups.
  3. Treat each lower group as nested within the preceding one, not as an unrelated label.
  4. Use the evidence appropriate to the rank; do not substitute a kingdom name for a species identification.

Domain is the highest rank, while species is the lowest in this hierarchy. The card establishes nested classification; detailed kingdom features and virus classification are separate cards.

Four kingdoms have distinct feature patterns

Protoctista, Fungi, Plantae and Animalia are kingdoms within Eukarya. Classify them from a pattern of cell structure, body organisation and nutrition rather than from one feature alone.

Kingdom Characteristic feature pattern
Protoctista mostly unicellular, though some are multicellular; highly varied; some are photosynthetic with chloroplasts, while others are heterotrophic; cell walls occur in some groups
Fungi unicellular or multicellular; no chloroplasts; cell walls contain chitin; heterotrophic by extracellular digestion and absorption; multicellular forms have hyphae forming a mycelium and commonly reproduce by spores
Plantae multicellular; cellulose cell walls and chloroplasts; autotrophic by photosynthesis; cells form differentiated tissues and organs
Animalia multicellular; no cell walls or chloroplasts; heterotrophic by ingestion; specialised cells form tissues and organs

Compare several features together. A cell wall does not by itself mean Plantae: fungal walls contain chitin, plant walls contain cellulose, and some protoctists also have walls. Likewise, both fungi and animals are heterotrophic, but fungi digest externally and absorb products whereas animals ingest food.

Fungi are not plants: they lack chloroplasts, do not photosynthesise and have chitin rather than cellulose in their cell walls. Protoctista is especially diverse, so not every member has every listed feature.

Viruses can be classified by nucleic acid

Viruses are acellular and have no metabolism of their own, so they depend on host cells for replication. They are therefore outside the cellular three-domain and Eukaryotic-kingdom classifications.

Classify a virus by two genome features:
Nucleic-acid type — DNA or RNA.
Strand form — single-stranded or double-stranded.
These give four broad combinations: single-stranded DNA, double-stranded DNA, single-stranded RNA and double-stranded RNA.

Use the classification in this order:

  1. Identify whether the viral genome is DNA or RNA.
  2. Determine whether that genome is single-stranded or double-stranded.
  3. Report the combination as the virus classification; do not use the host organism to replace the genome evidence.
  4. Keep the boundary clear: this is a nucleic-acid classification of an acellular, host-dependent entity, not a domain or kingdom assignment.

A virus is not placed in Bacteria, Archaea, Eukarya or one of the four Eukarya kingdoms simply because of the cell it infects. This card stops at genome-based classification and does not add named virus examples or replication-cycle detail.

18.2 Biodiversity

Syllabus
9700–2028–2029
Topic
18.2
Level
A2

Ecosystem and niche describe different scales

An ecosystem is the interacting system of a community of organisms and the environment they live in. It includes biotic components, abiotic conditions, energy flow and nutrient cycling. A habitat is where a species lives; its niche is the role it plays there.

Ecosystem — a system scale: populations interact with one another and with physical and chemical conditions.
Habitat — a place boundary: where the organism is found.
Niche — a role-and-resource boundary: how the organism obtains energy, uses conditions and interacts with other species and the physical environment.

Niche overlap means two species use some of the same resources or conditions. The more their roles and resource use overlap, the greater the potential for competition; distinct resource use can allow different species to fit into the same ecosystem. This is a relationship-level interpretation, not a biodiversity count or an automatic proof of competition.

Use the terms in this order:

  1. Name the ecosystem as the interacting living-and-non-living system.
  2. Identify the habitat as the place occupied by a species.
  3. Describe the niche through energy use, physical conditions and interactions.
  4. Compare niches when explaining overlap, possible competition or how multiple species can occupy an ecosystem; keep measurement of biodiversity for a separate assessment.

A habitat is not a niche: location alone does not describe a species’ role. Ecosystem, habitat and niche are concepts rather than sampling methods; this card does not introduce quadrats, correlation statistics or Simpson’s index.

Biodiversity has three assessment levels

Biodiversity describes the range and variety of genes, species and habitats within a region. It can be assessed at three linked levels.

  • Ecosystem/habitat diversity — the number and range of different ecosystems or habitats in the area.
  • Species diversity — the number of different species and how evenly individuals are distributed among them.
  • Genetic diversity — the variety of genes and alleles within each species, including differences between populations of the same species.

Species richness is only the number of species. Species diversity also considers evenness: an area can contain many species but still have lower species diversity if most individuals belong to one or two species. Keep these terms separate when interpreting evidence.

Biodiversity can support ecosystem resilience because variation provides more ways for populations and ecosystems to respond to environmental change. This is a general ecological relationship, not a guarantee that every diverse ecosystem resists every disturbance.

Biodiversity is broader than a species count: ecosystem/habitat, species and genetic levels answer different questions. This card defines the assessment levels; random sampling, field methods, correlation tests and Simpson’s index are separate cards.

Random sampling reduces investigator bias

Random sampling gives every possible sampling position an equal chance of selection. This prevents the investigator choosing unusually species-rich, accessible or visually interesting places and therefore reduces selection bias.

  1. Mark out the study area as a grid and assign coordinates.
  2. Use a random-number generator or random-number table to select coordinate pairs.
  3. Place the same sampling unit at each selected position and use the same identification and counting rules.
  4. Take many independent samples across the area and calculate the required abundance or diversity measure.

A less biased sample is more likely to represent the whole area, so an estimate can be generalised with greater confidence. Repetition also reduces the influence of an unusual sampling point and allows variation between samples to be assessed.

Random selection reduces bias; it does not guarantee that one or a few samples are representative. The study still needs an adequate sample size, a clearly defined area and standardised methods.

Match biodiversity methods to organisms and patterns

Choose a method from the organism's mobility and the evidence needed: quadrats estimate abundance in fixed areas, transects show distribution across a gradient, and mark-release-recapture estimates a mobile population.

Method Best use Record
Frame quadrat plants or slow/sessile organisms across an area species identity plus count, frequency or percentage cover in a known area; use random positions and repeats
Line transect presence and distribution along an environmental gradient species touching the line, continuously or at fixed points
Belt transect quantitative change in abundance across a gradient quadrats continuously or at regular intervals along a line
Mark-release-recapture mobile animals in a defined population first catch marked and released; after mixing, count total and marked individuals in a second catch

N = \frac{n_1 n_2}{m_2}

Here NN is estimated population size, n1n_1 is the first sample, n2n_2 is the whole second sample and m2m_2 is marked animals recaptured. If 27 frogs are marked, 33 are caught later and 13 are marked, N=(27×33)/13=68.5N=(27\times33)/13=68.5, reported as 69 animals.

The Lincoln estimate assumes marks are harmless, retained and recognised; marked animals mix fully and are as likely to be recaptured as others; and the population is effectively closed to births, deaths, immigration and emigration between samples. Repeat sampling and standardise effort.

Choose Spearman or Pearson for paired ecological data

Spearman's rank correlation and Pearson's linear correlation test whether paired values of two variables are associated. Either can relate a biotic measurement, such as species abundance, to an abiotic factor, such as altitude or temperature.

Test Use when What the coefficient measures
Spearman's rsr_s data can be ranked; the relationship is monotonic; normality or a linear pattern is not required strength and direction of rank association
Pearson's rr both variables are quantitative, the relationship is approximately linear and assumptions including normality are met strength and direction of linear association
  1. Pair measurements from the same sampling units and plot a scatter graph.
  2. State the null hypothesis: there is no correlation between the variables.
  3. Choose the test, then use the supplied formula to calculate rsr_s or rr.
  4. Compare the magnitude of the calculated coefficient with the critical value for sample size and probability level.
  5. If it meets or exceeds the critical value, reject the null hypothesis and report the direction in biological context; otherwise do not reject it.

Coefficients range from −1-1 to +1+1: the sign gives direction and values nearer either extreme indicate stronger association. Statistical significance is not causation; another variable or the sampling design may explain the pattern.

Simpson's index combines richness and evenness

Simpson's index of diversity, DD, combines species richness with relative abundance. Diversity is higher when more species are present and individuals are distributed more evenly among them.

D = 1 - \sum \left(\frac{n}{N}\right)^2

nn = number of individuals of one species.
NN = total number of individuals of all species in the sample.
Calculate (n/N)2(n/N)^2 for every species, add these values, then subtract the sum from 1.

A sample contains 6 individuals of species A, 3 of B and 1 of C, so N=10N=10. D=1−[(6/10)2+(3/10)2+(1/10)2]=1−(0.36+0.09+0.01)=0.54D=1-[(6/10)^2+(3/10)^2+(1/10)^2]=1-(0.36+0.09+0.01)=0.54. A community dominated by one species would have a lower value.

DD ranges from 0 towards 1. A value closer to 1 indicates greater diversity; a value near 0 indicates low diversity or strong dominance. Compare samples collected with compatible methods and effort: a higher DD does not identify which species caused the difference.

18.3 Conservation

Syllabus
9700–2028–2029
Topic
18.3
Level
A2

Four pressures can drive populations to extinction

A species becomes extinct when no living individuals remain. Extinction risk rises when a pressure repeatedly lowers survival or reproductive success so that deaths exceed births and the population can no longer recover.

Pressure Route to extinction
Climate change temperature, rainfall, sea level or ocean conditions shift beyond tolerance; habitat, food or breeding conditions are lost
Competition another population uses the same limited food, space, light, water or mates more successfully, reducing survival or reproduction
Hunting by humans individuals are removed faster than reproduction replaces them, especially when breeding adults are taken
Habitat degradation or loss pollution, fragmentation or destruction reduces usable area, resources, shelter and access to mates

Pressures can reinforce one another. Habitat loss may crowd organisms into smaller areas and increase competition. As a population shrinks, loss of genetic variation and inbreeding can reduce its ability to respond to further change, increasing the chance that the decline continues.

A fall in abundance is not yet extinction, and one pressure need not act alone. Explain the complete chain from the named pressure to reduced survival or reproduction, population decline and eventual loss of the species.

Biodiversity has ecological and human value

Maintaining biodiversity protects ecological relationships and preserves options for people and other species. The reasons overlap: ecosystems, species and genes support one another, so loss at one level can reduce benefits at others.

  • Ecological stability and resilience — more diverse ecosystems may contain species able to tolerate environmental change, while loss of a keystone species can disrupt many other organisms.
  • Environmental services — plants, fungi, bacteria and other organisms contribute to carbon dioxide absorption, the water cycle, decomposition, nutrient cycling and food webs.
  • Genetic resources and future value — wild relatives can provide alleles that help crops resist disease or other disasters, and organisms may contain medicines or useful materials that have not yet been discovered.
  • Economic value — biodiversity supports medicines, ecotourism, employment, science and technology.
  • Social, cultural and aesthetic value — natural environments provide recreation, inspiration and opportunities for people to connect and learn.
  • Moral and ethical value — people may regard humans as responsible for reducing human-caused loss and valuing other species.

These reasons are interdependent rather than isolated: genetic and species diversity can support ecosystem resilience; functioning ecosystems provide services and resources; and those benefits affect human wellbeing and future choices. The evidence supports a general reason to maintain biodiversity, not a guarantee that every diverse ecosystem resists every disturbance.

Do not reduce conservation value to economic usefulness alone, and do not assume that an ecosystem service or future genetic resource is certain or unlimited. This card explains why biodiversity matters; conservation methods, assisted reproduction, invasive-species control and IUCN/CITES tools are separate cards.

Conservation can be in situ or ex situ

In situ conservation protects a species in its natural habitat. Ex situ conservation protects organisms or their genetic material outside the original habitat when in situ protection is not sufficient or possible.

  • Protected areas (in situ) — national and marine parks protect habitats and species through controls on access, development, hunting, overfishing or pollution; the natural support systems remain available.
  • Zoos and botanic gardens (ex situ) — captive populations support conservation, research and education, and may provide material for future reintroduction; suitable conditions and genetic diversity must be maintained.
  • Frozen zoos and gene banks (ex situ) — animal eggs, sperm or tissues can be stored at very low temperature to preserve genetic material from different individuals.
  • Seed banks (ex situ) — dried seeds are stored under controlled conditions to conserve plant genetic diversity and allow future regrowth; some plants require continued growth or tissue culture instead of freezing.

Choose the method to match the threat and biological need. In situ protection maintains the whole habitat but depends on effective management and community acceptance. Ex situ storage or captivity can protect a small or isolated population, but it costs resources, may not reproduce the natural habitat, and small captive populations can lose genetic diversity. Long-term success also depends on a suitable habitat or use for the conserved material.

In situ and ex situ methods are complementary, not interchangeable. This card compares conservation settings and genetic-resource storage; assisted-reproduction procedures, invasive-species control and IUCN/CITES roles are separate cards.

IVF creates embryos; transfer and surrogacy support gestation

Assisted reproduction can produce offspring when endangered mammals have few suitable mates or reproduce poorly in captivity. Genetic records guide the choice of parents so that breeding can maintain variation and limit inbreeding.

Method What happens Conservation role
IVF hormones stimulate egg maturation; eggs are collected, mixed with sperm outside the body, and fertilised embryos are cultured briefly gametes from genetically suitable animals can be combined without natural mating
Embryo transfer an early embryo is placed into the uterus of a prepared recipient female a donor's embryo can complete development in another female, allowing valuable genes to produce more offspring
Surrogacy the recipient female carries the transferred embryo through pregnancy and gives birth a surrogate can carry an embryo when the genetic mother should not or cannot undergo pregnancy

In a typical IVF programme: stimulate egg maturation → collect eggs and sperm → fertilise outside the body → check early embryos → transfer a selected embryo to a recipient uterus → monitor pregnancy and birth. The offspring's nuclear genes come from the egg and sperm donors, not from the surrogate.

The three terms describe linked but different stages: IVF creates the embryo, embryo transfer moves it, and surrogacy is gestation by a recipient. These methods do not restore habitat or automatically solve low genetic diversity, cost, welfare or post-release survival.

Control invasive alien species through prevention and evidence

An invasive species is a species that has moved into an ecosystem where it was previously unknown and then disrupts the balance of the ecosystem. Most recorded introductions are linked to human transport, trade or deliberate release.

  1. Identify the introduced species, the route by which it arrived and the native species or ecosystem processes affected.
  2. Monitor its spread and population increase, together with competition, predation, disease and changes in biodiversity or productivity.
  3. Prevent further introduction or spread by controlling the human pathways that transport species between ecosystems; deliberate biological-control introductions also require evidence because a non-native species may become invasive.
  4. If control is considered, judge the intervention against reduction of the invasive population and recovery of native species, while checking non-target effects, ecosystem disruption, cost and ethical acceptability.
  5. Continue long-term monitoring: a short-term fall in numbers is not the same as lasting recovery of the native community.

The risk chain is: few natural predators, competitors or pathogens → rapid population increase → competition for prey, nutrients, light or space, successful predation or introduction of disease → native-population decline and reduced biodiversity/productivity. Human health, travel and economic costs can also be affected.

Do not call every non-native species invasive, and do not assume that one control action is automatically effective or harmless. The matched SME page does not specify a physical, chemical or biological control protocol, so no named treatment, dose or non-target claim is added here. Assisted reproduction and IUCN/CITES roles are separate cards.

IUCN assesses risk; CITES regulates international trade

IUCN and CITES support conservation with different tools. IUCN provides evidence-based assessments of extinction risk; CITES is an international agreement that controls trade in listed wild animals, plants and their products.

Organisation Main role How this helps conservation
IUCN assesses population size and trend, distribution, threats and extinction risk; publishes conservation-status categories in the Red List identifies and prioritises species at risk, informs governments and conservation organisations, raises awareness and supports planning; assessments are updated as evidence changes
CITES lists species in appendices and regulates or prohibits their international commercial trade; permitted trade requires documentation and monitoring reduces unsustainable legal trade and illegal international trade, gives countries a shared framework, and makes cross-border enforcement and trade records possible

The tools can complement each other: an IUCN assessment may show that trade contributes to risk, while CITES controls the international trade route. A species can still receive CITES protection even when IUCN data are insufficient, because the organisations use different processes.

An IUCN Red List category is not itself a law or trade ban. CITES regulates international trade; it does not assign Red List categories or directly remove every threat such as habitat loss, climate change or domestic exploitation.