Course review

A2.2 Cell structure

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Learning objective

A2.2.1—Cells as basic structural unit

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• Cells are the basic structural and functional units of living organisms • Cells carry out essential life processes and are usually microscopic

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A2.2.2—Microscopy skills

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• Use light microscopes to prepare mounts, stain specimens, and observe cells • Measure cells with eyepiece graticules and stage micrometers • Calculate magnification, actual size, and scale bars

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A2.2.3—Developments in microscopy

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• Resolution, not only magnification, determines visible detail • TEM reveals internal ultrastructure; SEM reveals surface detail • Cryogenic EM, fluorescence, and immunofluorescence reveal molecules and specific structures

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A2.2.4—Structures common to all cells

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• All cells have a plasma membrane, cytoplasm, DNA, and ribosomes • These structures control exchange, support metabolism, store information, and make proteins

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A2.2.5—Prokaryote cell structure

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• Prokaryotes lack a nucleus and membrane-bound organelles • Structure includes cell wall, plasma membrane, cytoplasm, 70S ribosomes, naked circular DNA, and plasmids • E. coli, Bacillus, and Staphylococcus are bacterial examples

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A2.2.6—Eukaryote cell structure

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• Eukaryotes have a nucleus, 80S ribosomes, cytoskeleton, and compartmentalized cytoplasm • Organelles include mitochondria, ER, Golgi apparatus, vesicles, lysosomes, and vacuoles • Plant cells may include chloroplasts, large vacuoles, and cellulose cell walls

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A2.2.7—Processes of life in unicellular organisms

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• Unicellular organisms carry out all life processes in one cell • Examples include Amoeba, Chlamydomonas, and Escherichia coli • Processes include nutrition, metabolism, response, excretion, homeostasis, growth, and reproduction

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A2.2.8—Differences in eukaryotic cells

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• Animal, fungal, and plant cells differ in cell walls, vacuoles, and storage structures • Plant cells have cellulose walls, chloroplasts, and large permanent vacuoles • Animal cells may have centrioles, cilia, flagella, lysosomes, and temporary vacuoles

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A2.2.9—Atypical cell structure

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• Atypical cells show limits of simple cell theory • Examples include multinucleate fungal hyphae and striated muscle fibres • Red blood cells and phloem sieve tubes lack nuclei at maturity

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A2.2.10—Cell identification in micrographs

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• Identify prokaryotic, plant, and animal cells in light and electron micrographs • Recognize organelles such as nucleus, mitochondria, chloroplasts, ER, Golgi, vacuoles, and ribosomes • Use visible structures, scale, and tissue context as evidence

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A2.2.11—Drawing and annotation

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• Draw clear biological diagrams from electron micrographs without shading • Label visible organelles and include magnification or scale information • Annotate structures with functions where required

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